Coding
Knt-GFP

Part:BBa_K3427001:Design

Designed by: Clementine Sejotte and Florent Poubanne   Group: iGEM20_GO_Paris-Saclay   (2020-10-20)


knt-gfp entangled CDSs for aminoglycoside resistance and GFP


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 784
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 784
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 784
    Illegal BamHI site found at 378
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 784
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 784
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

BsaI sites were added to allow a Golden Gate after the entangled gene given by CAMEOS

Source

This part was designed with a software named Cameos (Thomas Blazejewski et al, Science ,09 Aug 2019:Vol. 365, Issue 6453, pp. 595-598, DOI: 10.1126/science.aav5477) to entangle this 2 coding genes : Knt and GFP

References